DNA Sequencing Core Facility
The Biochemistry Biotechnology Facility offers an automated DNA sequencing service which can be used for sequence analysis of plasmid, PCR, and large DNA samples. This service utilizes two Perkin Elmer / Applied Biosystems 3100 Genetic Analyzers and Big Dye Terminator chemistry v3.1. We routinely provide between 700 to 1000 bases per reaction providing the template is of high quality.
The turn-around time for DNA sequence data is generally 24 hours from sample submission if all sample requirements are met. Data are supplied as a hard copy electrophoretograph, if requested, and an electronic text file. Electrophoretograph and text data files can be retrieved electronically from this web site by researchers at Indiana University. For questions or more detailed discussion about this service, please contact Sequencing personnel.