B.S., 1986, Sichuan University, Chengdu, Sichuan, China
M.S., 1993, Oregon State University, Corvallis, OR
Ph.D., 1998, Washington University, St. Louis, MO
Post Doctoral, 1998-2001, Indiana University School of Medicine, Indianapolis, IN

Area of Study

The use of proteomic technologies such as label-free mass spectrometry-based shotgun protein quantification and multiple-reaction-monitoring (MRM) for biomarker development.   More details...

Selected Publications

Johnson, D. T.; Harris, R. A.; French, S.; Blair, P. V.; You, J-S.; Bemis, K. G.; Wang, M.; Balaban, R. S. (2006) The Tissue Heterogeneity of the Mammalian Mitochondrial Proteome. Am J Physiol Cell Physiol, 291, C689-C697.

Brown, D. P. G.; Gokmen-Polar, Y.; Jiang, L.; Tan, J.; Ringham, H.; Janecki, D. J.; Qi, G.; Witzmann, F. A.; Sledge, G. W. Jr.; Wang, M. (2007) A comparative proteomic study to characterize the vinblastine resistance in human ovarian cancer cells. Proteomics - Clinical Applications. 1, 18-31.

Fitzpatrick, D. P. G.; You, J-S.; Bemis, K. G.; Wery, J-P.; Ludwig, J. R.; Wang, M.; (2007) Searching for Potential Biomarkers of Cisplatin Resistance in Human Ovarian Cancer Using a Label-free LC/MS-based Protein Quantification Method. Proteomics - Clinical Applications. 1, 246-263.

Chen, J. Y.; Yan, Z.; Shen, C.; Fitzpatrick, D. P. G.; Wang, M.; (2007) A Systems Biology Approach to the Study of Cisplatin Drug Resistance in Ovarian Cancer. J Bioinfo Compu Biol. 5, 383-405.

Janecki, D. J.; Bemis, K. G.; Tegeler, T. J.; Sanghani, P. C.; Zhai, L.; Hurley, T. D.; Bosron, W. F.; Wang, M. (2007) A Multiple Reaction Monitoring Method for Absolute Quantification of the Human Liver Alcohol Dehydrogenase ADH1C1 Isoenzyme. Anal Biochem, 366, 18-26.

Dey, N; De, P. K.; Wang, M.; Zhang, H.; Dobrota, E. A.; Robertson, K. A.; Durden, D. L. (2007) CSK Controls Retinoic Acid Receptor Signaling: RAR-cSRC Signaling Axis is Required for Neuritogenic Diffentiation. Mol Cell Biol, 27, 4179-4197.

Li, X.; Wang, S.; Qi, J.; Echtenkamp, S. F.; Chatterjee, R.; Wang, M.; Boons, G-J.; Dziarski, R.; and Gupta, D. (2007) Zebrafish Peptidoglycan Recognition Proteins Are Bactericidal Amidases Essential for Defense against Bacterial Infections. Immunity, 27, 518-529.

Conner, S. L. and Wang, M. (2008) Identification of FANCA Interacting Proteins in Mammalian Cells Using Tandem Affinity Purification and Mass Spectrometry. Scientific Research and Essays, 3, 143-153.

Wang, M.; You, J-S.; Bemis, K. G.; Tegeler, T. J.; Brown, D. P. G. (2008) Label-free Mass Spectrometry-based Protein Quantification Technologies in Proteomic Analysis. Briefings in Functional Genomics and Proteomics, 7, 329-339.

Brown, D. P. G.; Chin-Sinex, H.; Nie, B.; Mendonca, M. S.; Wang, M. (2008) Targeting Superoxide Dismutase 1 to Overcome Cisplatin Resistance in Human Ovarian Cancer. Cancer Chemotherapy and Pharmacology, 63, 723-730.

Huang, S. K.; Darfler, M. M.; You, J-S.; Bemis, K. G.; Tegeler, T. J.; Wang, M.; Wery, J-P.; Chong, K. K.; and Hoon, D. S. B. (2009) LC/MS-based Quantitative Proteome Analysis of Paraffin-Embedded Archival Melanoma Tissues Reveal Potential Proteomic Biomarkers Associated with Metastasis. PLoS ONE, 4, e4430, 1-12.

McBride, W. J.; Schultz, J. A.; Kimpel, M. W.; McClintick, J. N.; Wang, M.; Rodd, Z. A. (2009) Differential Effects of Ethanol in the Nucleus Accumbens Shell of Alcohol-Preferring, Alcohol-Non-Preferring and Wistar Rats: A Proteomics Study. Pharmacology, Biochemistry and Behavior, 92, 304-313.

Addona, T.; Abbatiello, S. E.; Skates, S. J.; et al. (2009) A Multi-site Assessment of Precision and Reproducibility of Multiple Reaction Monitoring-based Measurements by the NCI-CPTAC Network: Toward Quantitative Protein Biomarker Verification in Human Plasma. Nature Biotechnology, 27, 633-641.

Nakshatri, H.; Qi, G.; You, J.; Bemis, K.; Schneider, B.; Zon, R.; Buck, C.; Regnier, F.; Wang, M. (2009) Intrinsic Subtype Associated Changes in Plasma Proteome in Breast Cancer. Proteomics - Clinical Applications, 3, 1305-1313.

Dzieciatkowska, M; Copeland, M; You, J; Wery, J-P; Wang, M. (2009) Comparison of an enzyme immunoassay versus mass spectrometry-based assay for the quantitative determination of the procollagen type I N-terminal propeptide in rat serum. Proteomics Insights, 2, 33-38.

Rao, N; Jhamb, D; Milner, D. J; Li, B; Song, F; Wang, M; Voss, S. R; Palakal, M; King, M. W; Saranjami, B; Nye, H. L. D; Cameron, J. A.; and Stocum, D. L. (2009) Proteomic Analysis of Blastema Formation in Regenerating Axolotl Limbs. BMC Biology, 7, 83.

Richardson, M. R; Price, M. O; Price, F. W; Pardo, J. C; Grandin, J. C; You, J; Wang, M; Yoder, M. C. (2009) Proteomic Analysis of Human Aqueous Humor Using Multidimensional Protein Identification Technology. Mol Vision, 15, 2740-2750.

Bell, L. N.; Theodorakis, J.; Vuppalanchi, R.; Saxena, R.; Bemis, K. G.; Wang, M., Chalasani, N. (2010) Serum Proteomics and Biomarker Discovery across the Spectrum of Nonalcoholic Fatty Liver Disease. Hepatology, 51, 111-120.

Paulovich, A. G; Billheimer, D; Ham, A. J; et al. (2010) A CPTAC inter-laboratory study characterizing a yeast performance standard for benchmarking LC-MS platform performance. Molecular & Cellular Proteomics, 9, 242-254.

Rudnick, P. A; Clauser, K. R; Kilpatrick, L. E; et al. (2010) Performance metrics for liquid chromatography-tandem mass spectrometry systems in proteomic analyses and evaluation by the CPTAC network. Molecular & Cellular Proteomics, 9, 225-241.

Tabb, D. L; Vega-Montoto, L; Rudnick, P; et al. (2010) Repeatability and Reproducibility in Proteomic Identifications by Liquid Chromatography-Tandem Mass Spectrometry. J Proteome Res, 9, 761-776.

Kim, J; Nie, B; Sahm, H; Brown, D. P. G; Tegeler, T; You, J; Wang, M. (2010) Targeted Quantitative Analysis of Superoxide Dismutase 1 in Cisplatin-sensitive and Cisplatin-resistant Human Ovarian Cancer Cells. J Chromatogr B Analyt Technol Biomed Life Sci, 878, 700-704.

Zhang, X; Fan, A; Riley, C. P; Buck, C; Wang, M; Regnier, F. E. (2010) Multi-dimensional Liquid Chromatography in Quantitative Proteomics. Analytica Chimica Acta, 664, 101-113.

Bell, L. N; Lee, L; Saxena, R; Bemis, K. G; Wang, M; Theodorakis, J; Vuppalanchi, R; Alloosh, M; Sturek, M; Chalasani, N. (2010) Serum Proteomic Analysis of Diet-Induced Steatohepatitis and Metabolic Syndromes in the Ossabaw Miniature Swine. Am J Physiol Gastrointest Liver Physiol, 298, G746-G754.

Jiang, Y. and Wang, M. (2010) Personalized Medicine in Oncology: Tailoring the Right Drug to the Right Patient. Biomarkers in Medicine, 4(4), 523-533.

Kim, J; Sahm, H; You, J; Wang, M. (2010) Knock-down of Superoxide Dismutase 1 Sensitizes Cisplatin-resistant Human Ovarian Cancer Cells. Anticancer Res, 30(7), 2577-2581.

Shao, C. H; Rozanski, G. J; Nagai, R; Stockdale, F. E; Patel, K. P; Wang, M; Singh, J; Mayhan, W. G; Bidasee, K. R. (2010) Carbonylation of myosin heavy chains in rat heart during diabetes. Biochem Pharmacol, 80, 205-217.

Zhang, F; Wang, M; Chen, J. Y. (2010) Breast Cancer Plasma Protein Biomarker Discovery by Coupling LC-MS/MS Proteomics and Systems Biology. ACM-BCB 2010.

Shao, C. H; Capek, H. L; Patel, K. P; Wang, M; Tang, K; DeSouza, C; Nagai, R; Mayhan, W; Periasamy, M; Bidasee, K. R. (2011) Carbonylation Contributes to SERCA2a Activity Loss and Diastolic Dysfunction in a Rat Model of Type 1 Diabetes. Diabetes, 60, in press.

Dzieciatkowska, M; Qi, G; You, J; Bemis, K. G; Sahm, H. M; Lederman, H. M; Gelbert,  L. M; Wang, M. (2011) Proteomic Characterization of Cerebrospinal Fluid of Ataxia-telangiectasia Patients. Intl J Proteomics, in press.
 
Bell, L. N; Saxena, R; Mattar, S. G; You, J; Wang, M; Chalasani, N. (2011) Utility of fixed, paraffin-embedded liver biopsy material for proteomic profiling: direct comparison with frozen liver tissue. Proteomics – Clinical Applications, in press.

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Research Interests

Chemotherapy is still the primary treatment for many types of cancer. Most cancer patients with the disease are initially responsive to chemotherapeutic treatment. However, drug resistance has become a major impediment to the successful treatment of cancer. To date the mechanism(s) of drug resistance are yet fully understood. Previous studies have suggested that many proteins, such as BRCA1, BRCA2, MDR1, MRP1, MDM2, hMLH1, HSP27, and HSP70, are differentially expressed in drug-resistant tumor cells, such as ovarian tumor cells, by mRNA differential display analysis. Using multiple proteomic platforms, we have identified a panel of potential protein targets that can serve as biomarkers for drug resistance diagnosis and targets for new therapeutic development. My lab is focusing on the use of the cutting-edge proteomic technologies such as MudPIT, label-free protein quantification, and MRM-based protein assays to identify and validate protein biomarker candidates that are the causing factors for certain biological behaviors. Other areas of my research are to develop non antibody-based, high-throughput mass spectrometry-based assays that could quantitatively measure biomarkers from a variety of species in multiplexed fashion and to develop early cancer diagnostic biomarker panels for breast and ovarian cancers.