Assistant Professor

Department of Biochemistry and Molecular Biology
Indiana University School of Medicine
John D. Van Nuys Medical Science Building
635 Barnhill Drive, room MS1021H
Indianapolis, Indiana 46202-5126

Phone: (317) 278-2350
Facsimile: (317) 274-4686
E-mail: almosley@iupui.edu

B.S. in Biology/Philosophy, 1995, East Tennessee State University
Ph.D. in Biochemistry, 2004, University of Kentucky College of Medicine
Postdoctoral fellow, 2004-2010, Stowers Institute for Medical Research

Area of Study

Analysis of protein-protein interaction networks and post-translational modifications that regulate RNA Polymerase II transcription using protein mass spectrometry and genomics.   More details...

Selected Recent Publications

Mosley, A.L., Sardiu, M.E., Pattenden, S.G., Workman, J.L., Florens, L., Washburn, M.P.. Highly reproducible label free quantitative proteomic analysis of RNA polymerase complexes. Molecular & Cellular Proteomics. 2011 Feb;10(2):M110.000687

Fournier, M.L., Paulson, A., Pavelka, N., Mosley, A.L., Zueckert-Gaudenz, K., Bradford, W.D., Glynn, E., Li, H., Sardiu, M.E., Seidel, C., Florens, L., and Washburn, M.P. Delayed Correlation of mRNA and Protein Expression in Rapamycin Treated Cells and a Role for Ggc1 in Cellular Sensitivity to Rapamycin. Molecular & Cellular Proteomics. 2010 Feb;9(2):271-84.

Mosley, A.L., Pattenden, S.G., Carey, M., Venkatesh, S., Gilmore, J.M., Florens, L., Workman, J.L., Washburn, M.P. Rtr1 is a CTD phosphatase that regulates RNA polymerase II during the transition from serine 5 to serine 2 phosphorylation. Molecular Cell. 2009. 34(2):168-178.

Mosley, A.L., Florens, L., Wen, Z., Washburn, M.P. A label free quantitative proteomic analysis of the Saccharomyces cerevisiae nucleus. J Proteomics. 2009 Feb 15. 72(1):110-20.

Black, J.C., Mosley, A.L., Kitada, T. Washburn, M.P., Carey, M. The SIRT2 deacetylase regulates autoacetylation of p300. Molecular Cell. 2008. 32(3):449-455.

Zybailov, B.L., Mosley, A.L., Sardiu, M.E., Coleman, M.K., Florens, L., and Washburn, M.P. Statistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae. J. Proteome Res. 2006 Sep. 5(9):2339-47.

Mosley, A. L. and Özcan, S. Pdx-1 Interacts with Histone Deacetylases Hdac-1 and Hdac-2 on Low Levels of Glucose. J Biol Chem. 2004. 279(52):54241-54247.

Mosley, A. L. and Özcan, S. Glucose regulation of insulin gene expression requires the recruitment of p300 by the beta-cell specific transcription factor Pdx-1. Molecular Endocrinology. 2004. 18(9):2279-2290.

Mosley, A.L. and Özcan, S. Glucose Regulates Insulin Gene Transcription by Hyperacetylation of Histone H4. J. Biol. Chem. 2003. 278(22): 19660-19666

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Research Interests

In multiple disease states ranging from cancer to viral infection, the mechanisms of general transcription are altered. In order to understand the extent of disease manipulation of the transcription machinery, general mechanisms that control transcription under normal conditions must be understood. We apply quantitative proteomic and genomic techniques to study the dynamics of RNA Polymerase II transcription. Using Multidimentional Protein Indentification Technology (MudPIT), we can study both protein-protein interaction networks and post-translational modifications that may alter transcription during different cellular states.

During RNA Polymerase II transcription, the C-terminal domain (CTD) of Rpb1 has been proposed to act as a scaffold to coordinate transcription initiation, elongation, termination, histone modification, and mRNA processing events. These events have been shown to correlate with distinct changes in the pattern of CTD phosphorylation across open reading frames. The major focus of our research is to study the role of the CTD phosphatase Rtr1 during the transcription cycle and to understand how alterations in the phosphorylation state of the CTD influence gene expression and mRNA processing.